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Amit Singh

PROJECT AFFILIATION:

PROFESSIONAL ROLES

  • Higher Ed: Science, Researcher 

BIO

Drosophila eye model to study axial patterning, cell survival & birth defects.
The fruit fly, Drosophila melanogaster, eye serves as an excellent model to study cell type specification during development. Drosophila eye has been extensively used to address diverse biological processes like patterning cell proliferation, cell death, cell survival, polarity and genetic basis of human diseases. The compound eye of an adult fly develops from the primordium called eye imaginal disc harbored inside larva, which initiates from a group of 20- cells as early as an embryo (Fig. 1).

Fig.1 The compound eye of Drosophila comprising of 800 unit eyes, ommatidia, initiates from eye-antennal (E.A.) primordium present in the embryo (A), which grows into larval E.A imaginal disc (B). E.A imaginal disc give rise to adult eye (C), which comprises of dorsal (red) and ventral (blue) compartments antenna (green) and adult head structures. (Dorsal up and ventral down). 

EXPERTISE

Molecular Genetics of Drosophila
Cell Biology
Cell Death and Cell Proliferation
Growth and Patterning
Drosophila eye development
Drosophila eye model to understand neurodegeneration and cell survival 

RECENT PUBLICATIONS

PEER-REVIEWED PUBLICATIONS (IN CHRONOLOGICAL ORDER).

1. Kango-Singh, M. and Singh, A.* (2009). Regulation of Organ Size: Insights from the Drosophila Hippo signaling pathway. Dev. Dynamics (in press)
[* Co-Corresponding Author]

2. Singh, A., Kango-Singh, M., and Gopinathan, K.P. (2009). Patterning defects in silkworm embryos analysed through cuticle preparations. Sericologia (in press)
[* Corresponding Author]

3. Tare, M. and Singh, A. (2009). A cell Biology laboratory exercise to study sub-cellular organelles in Drosophila. D.I.S. (91): 160-164.
[* Corresponding Author]

4. Kango-Singh, M., Call, G. B., and Singh, A.*, (2008). Annual Drosophila meeting at San Diego 2008. Dev. Dynamics 237(11):3444-52.
[* Corresponding Author]

5. Lim, J. Lee, O.K. Hsu, Y.-C, Singh, A. and Choi, K.-W. (2007b) Drosophila TRAP230/240 are essential coactivators for Atonal in retinal neurogenesis. Dev. Biol. 308(2): 322-330

6. Singh, A., Kango-Singh, M., Parthasarathy, R. and Gopinathan, K.P. (2007a). Larval legs of mulberry silkworm Bombyx mori are prototypes for the adult legs. Genesis 45 (4): 169-176
[Corresponding Author]

7. Singh, A. (2006b). Fly eye development in fly meeting at Houston. Int. J. Dev. Biol. 50: 659-663.
[Corresponding Author]

8. Singh, A., Xiao, S. and Choi, K.-W. (2006a). Lobe and Serrate, are required for cell survival during early eye development in Drosophila. Development 133, 4771-4781.
[Co-Corresponding Author]

9. Singh, A., Chan, J., Chern, J.J. and Choi, K.-W. (2005a). Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye. Genetics. 171(1):169-83. Epub 2005 Jun 23. PMID: 15976174

10. Singh, A., Kango-Singh, M., Choi, K.-W. and Sun, Y.H. (2004). Dorso-ventral asymmetric functions of teashirt in Drosophila eye development depend on spatial cues provided by early DV patterning genes. Mechanisms of Development 121: 365-370. PMID: 15110046

11. Singh, A., Choi, K.-W. (2003). Initial state of Drosophila eye before dorso-ventral specification is equivalent to ventral. Development 130: 6351-6360. PMID: 14623824

12. Kango-Singh, M., Singh, A. and Sun, Y.H. (2003). Eyeless collaborates with Hedgehog and Decapentaplegic signaling in Drosophila eye induction. Developmental Biology 256 (1):49-60. PMID: 12654291

13. Singh, A., Kango-Singh, M. and Sun, Y.H. (2002). Eye suppression, a novel function of teashirt, requires Wingless signaling. Development 129(18): 4271-80. PMID: 12183379

14. Kango-Singh, M.*, Singh, A.* and Gopinathan, K.P. (2001). The wings of Bombyx mori develop from larval discs exhibiting an early differentiated state: a preliminary report. Journal of Bioscience 26(2), 167-177. PMID: 11426053
[Equal first author]

15. Singh, A. and Gopinathan, K.P. (1998). Confocal microscopy, a powerful tool for biological research. Current Science 74(10):841- 851. (<a href="http://www.ias.ac.in/j_archive/currsci/74/10/841-851/viewpage.html)<p">http://www.ias.ac.in/j_archive/currsci/74/10/841-851/viewpage.html)<p</a>>

16. Singh, A. and Gopinathan, K.P. (1997). Study of gene expression in whole mount silkworm embryos using heterologous Drosophila antibodies. Current Science 72 (2): 214-219. (<a href="http://www.ias.ac.in/j_archive/currsci/72/3/214-218/viewpage.html)<p">http://www.ias.ac.in/j_archive/currsci/72/3/214-218/viewpage.html)<p</a>>

17. Bhojwani, J., Singh, A., Misquitta, L., Mishra, A. and Sinha, P. (1995). Search for Drosophila genes based on patterned expression of mini-white reporter gene in adult eyes. Roux's Archives of Developmental Biology, 205(3-4): 114-121. (<a href="http://www.springerlink.com/content/k4267w753752444m/)<p">http://www.springerlink.com/content/k4267w753752444m/)<p</a>>

18. Singh, A. (1995). Enhancer Trap Technique: A Novel Tool for Identification and Developmental Characterization of Genes of Drosophila. Current Science 68: 517-525. (<a href="http://www.ias.ac.in/j_archive/currsci/68/5/517-525/viewpage.html)<p">http://www.ias.ac.in/j_archive/currsci/68/5/517-525/viewpage.html)<p</a>>

19. Singh A., Kango, M. and Sinha, P. (1994). An improved method for chemical devitellinization of X-gal stained Drosophila embryos. IJEB 33, 150-152. PMID: 7538973

B. BOOK CHAPTERS

20. Singh, A., Lim, J., and Choi K.-W (2005b). Dorso-ventral boundary is required for organizing growth and planar polarity in the Drosophila eye. In "Planar Cell Polarization during Development: Advances in Developmental Biology and Biochemistry" (ed. M. Mlodzik) pp. 59-91, Elsevier Science & Technology Books.

21. Gopinathan, K.P., Joy, O., and Singh, A. (1998). In "Genome Analysis in Eukaryotes: Developmental and evolutionary aspects " (eds. R. M. Chatterjee and L. Sanchez) pp. 65-97, Springer-Verlag New York, Incorporated.

C. INVITED COMMENTARY & OTHERS
22. Singh, A.*, Kango-Singh, M (2006a). Annual Drosophila meeting at Houston 2006. Dev. Dynamics. (Published Online: 26 Jul 2006) PMID: 16871634 [Corresponding Author]

23. Singh, A., Kango, M. An improved devitellinization technique with a high yield of X-gal stained embryos. D. I. S. 1995 76:215.

D. MANUSCRIPT UNDER REVIEW

24. Singh, A. and Tsonis, P.A. (2009). Focus on molecule: Six 3. Experimental Eye Research